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| Untersuchte Arbeit: Seite: 44, Zeilen: 1-6 |
Quelle: Becker et al 1999 Seite(n): H2241, Zeilen: l.col: 21-31 |
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| [Intracellular oxidant stress can be monitored by measuring] changes in fluorescence resulting from intracellular probe oxidation. DHE enters the cell and is oxidized by ROS, particularly superoxide, to yield fluorescent ethidium. Ethidium binds to DNA (Eth-DNA), further amplifying its fluorescence. Eth-DNA fluorescence is generally stable but can be decreased by hydroxyl radical attack. Thus increases in DHE oxidation to Eth-DNA (i.e., increases in Eth-DNA fluorescence) are suggestive of superoxide generation. | Intracellular oxidant stress was monitored by measuring changes in fluorescence resulting from intracellular probe oxidation. Dihydroethidine (DHE, 1–10 μmol/l; Molecular Probes) enters the cell and is oxidized by ROS, particularly superoxide, to yield fluorescent ethidium. Ethidium binds to DNA (Eth-DNA), further amplifying its fluorescence (5). Eth-DNA fluorescence is generally stable but can be decreased by hydroxyl radical attack (27). Thus increases in DHE oxidation to Eth-DNA (i.e., increases in Eth-DNA fluorescence) are suggestive of superoxide generation.
5. Carter,W. O., P. K. Narayanan, and J. P. Robinson. Intracellular hydrogen peroxide and superoxide anion detection in endothelial cells. J. Leukoc. Biol. 55: 253–258, 1994. 27. Prutz, W. A. Inhibition of DNA-ethidium bromide intercalation due to free radical attack upon DNA. Radiat. Environ. Biophys. 23: 1–18, 1984. |
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