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| Untersuchte Arbeit: Seite: 38, Zeilen: 7-24 |
Quelle: Gangadharan 2006 Seite(n): 42, Zeilen: 6-21 |
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| Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and the dura was removed before transferring the brain into cooled and carbogenated (carbogen: gas consisting of 95% O2 and 5% CO2) artificial cerebrospinal fluid (ACSF) (temperature 40C) (Reymann et al., 1985). Cold solution was used to slow down the metabolism of the tissue, to limit the extent of excitotoxic and other kinds of damage occurring during the preparation of slices (Reymann et al., 1985). Cooling the petridish and tissue slicer support on ice may help to reduce tissue deterioration. Brain is placed in a petridish on filter paper and the cerebellum and frontal cortex is dissected away. Then the remaining part of the brain is divided in the central sulcus by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of a manuel chopper (Cambden, UK). The hippocampus was chopped into 400μm thick slices at 700 angle transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush floated in a glass vessel containing the cooled and carbogenated ACSF, and immediately transferred to the nylon net in the experimental chamber maintained at 320C by a wide mouthed pipette.
• Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15:249-255. |
Following decapitation, the skin and fur covering the skull were cut away and an incision was made on both sides. The bone covering the brain was prised away and dura removed before transferring the brain into chilled and carbogenated (carbogen: gas consisting of 95% O2 and 5% CO2) artificial cerebrospinal fluid (ACSF) (about 4°C) (Reymann et al., 1985). Cold solution was used to slow down the metabolism of the tissue, to limit the extent of excitotoxic and other kinds of damage occurring during the preparation of slices (Reymann et al., 1985). Chilling the petridish and tissue slicer support on ice may help reduce tissue deterioration. Brain is placed in a petriplate on filter paper and the cerebellum and frontal cortex is dissected away. Divide the remaining part of the brain in the central sulcus by a deep cut using a scalpel and the hippocampal commissure was cut and the right hippocampus was taken out on to the stage of manuel tissue chopper (Cambden, UK), and 400 μm thick slices were cut at 70° transverse to the long axis from the middle third of the right hippocampus. After sectioning, the slices were picked up by a wet artist’s brush, floated in a petri dish containing the cooled and carbogenated ACSF, and immediately transferred to the nylon net in the experimental chamber maintained at 32oC by a wide bored pipette.
133. Reymann KG, Malisch R, Schulzeck K, Brodemann R, Ott T, Matthies H (1985) The duration of long-term potentiation in the CA1 region of the hippocampal slice preparation. Brain Res Bull 15: 249-255. |
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