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| [1.] Iam/Fragment 045 01 - Diskussion Zuletzt bearbeitet: 2014-03-12 20:16:45 Graf Isolan | Fragment, Gesichtet, Iam, KomplettPlagiat, SMWFragment, Schutzlevel sysop, Zibrova 2004 |
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| Untersuchte Arbeit: Seite: 45, Zeilen: 1-12 |
Quelle: Zibrova 2004 Seite(n): 64, Zeilen: 5-17 |
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| 3.3.2.d Immunovisualization
After transfer, the membrane was incubated on the rocking platform with blocking solution overnight at 4°C. Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 2 h at room temperature. After washing (six times, five min on each occasion), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 h at room temperature. Afterwards, the membrane was washed as before. For the chemiluminescent detection SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 min at room temperature, laid between two sheets of transparent plastic protector and exposed to X-ray film, which was developed afterward according to the manufacturer’s instructions. |
Immunovisualization
After transfer, the membrane was incubated on a rocking platform with blocking solution overnight at 4°C (or alternatively, for 60 minutes at room temperature). Next, the membrane was incubated with primary antibody diluted in antibody dilution buffer for 1 hour at room temperature. After washing (six times five minutes each), the membrane was incubated with HRP-conjugated secondary antibody diluted in antibody dilution buffer for 1 hour at room temperature. Afterwards the membrane was washed as before. For the chemiluminescent detection, SuperSignal® West Pico Chemiluminescent Substrate (Pierce) was used. Substrate working solution was prepared by mixing of equal volumes of two substrate components. The membrane was incubated with substrate working solution for 5 minutes at room temperature, laid between sheets of transparent plastic protector and exposed to X-ray film, which was developed afterwards according to manufacturer’s instructions. |
Nearly identical but for "corrections" (and a minimal adaption). Nevertheless no part has been marked as a citation. |
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Letzte Bearbeitung dieser Seite: durch Benutzer:Graf Isolan, Zeitstempel: 20140312202043