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Quelle: Gu et al 2009
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Figure 13: Modulation of the dynamic equilibrium G- and Total actin in testosterone-HSA stimulated Caco2 cells.

24h serum starved cells were stimulated with 10 -7 M androgen conjugate for the indicated time points.

A) Total and G- actin were measured by quantitative immunoblot analysis after Triton X-100 subcellular fractionation. Bars present the G/Total actin mean value  SE of four independent duplicate experiments (** P < 0.01).

B) Cells were stained with rhodamine-phalloidin for filamentous actin and DRAQ5™ for nuclei.

Tubulin cytoskeleton reorganization was further analyzed by confocal laser scanning microscopy. A clear redistribution of the microtubular network became evident in cells treated with 10-7 M testosterone-HSA for 15 to 60 minutes (Fig. 14).

We further analyzed tubulin cytoskeleton reorganization by confocal laser scanning microscopy. A clear redistribution of the microtubular network became evident in cells treated with 10-7 M testosterone-HSA for 15 to 60 min (Additional File 1).

FMiogduurleat 3ion of the dynamic equilibrium between G- and Total actin in testosterone-HSA stimulated Caco2 cells Modulation of the dynamic equilibrium between G- and Total actin in testosterone-HSA stimulated Caco2 cells. 24 h serum starved cells were stimulated with 10 -7 M androgen conjugate for the indicated time points. (A) Total and Gactin were measured by quantitative immunoblot analysis after Triton X-100 subcellular fractionation. Bars present the G/Total actin mean value ± SE of four independent duplicate experiments (** P < 0.01). (B) Cells were stained with rhodamine-phalloidin for filamentous actin and DRAQ5™ for nuclei. Confocal laser scanning microscopy analyzed samples. Magnification, ×100.

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