von Dr. Vijay Pal Singh Rawat
Statistik und Sichtungsnachweis dieser Seite findet sich am Artikelende
| [1.] Vpr/Fragment 036 07 - Diskussion Zuletzt bearbeitet: 2014-04-04 23:00:51 Hindemith | Fontanari Krause 2006, Fragment, Gesichtet, SMWFragment, Schutzlevel sysop, Verschleierung, Vpr |
|
|
|
| Untersuchte Arbeit: Seite: 36, Zeilen: 7-22, 25-27 |
Quelle: Fontanari Krause 2006 Seite(n): 93, Zeilen: 3-18 |
|---|---|
| 3.12 BM transplantation and assessment of mice.
For bone marrow transplantation procedures, 8–10 week old recipient F1 (B6C3) mice were irradiated with 850 cGy from a 137Cs [sic] γ-radiation source. FACS purified transduced BM cells or defined ratios of transduced and untransduced cells were injected into the tail vein of irradiated recipient mice. PB or BM cell progeny of transduced cells were tracked using GFP or YFP fluorescence (Feuring-Buske et al., 2002). Lineage distribution was determined by flow cytometric analysis as previously described (Pineault et. al., 2003): Phycoerythrin-( PE) labeled Gr-1, Sca1, Ter-119, CD4 and allophycocyanin (APC) labeled Mac1, c-Kit, B220, CD8 antibodies were used for analysis (all Pharmingen Heidelberg, Germany). For histological analyses, sections of selected organs were prepared and H&E stained at the Academic Pathology Laboratory, GSF, Munich, using standard protocols. The mice were under observation for early signs of leukemia. Aspiration of the blood and bone marrow was performed at 8 weeks and subsequently at four week intervals and assessed for engraftment of the mouse with transduced cells and progression of disease. [...] In the animal, the indications of initiation of disease were paleness of the feet, limited mobility or lethargy, short breaths or ruffled body hair. Moribund mice were sacrificed and analyzed as described. Feuring-Buske, M., Frankel, A. E., Alexander, R. L., Gerhard, B., and Hogge, D. E. (2002). A diphtheria toxin-interleukin 3 fusion protein is cytotoxic to primitive acute myeloid leukemia progenitors but spares normal progenitors. Cancer Res 62, 1730-1736. Pineault, N., Buske, C., Feuring-Buske, M., Abramovich, C., Rosten, P., Hogge, D. E., Aplan, P. D., and Humphries, R. K. (2003). Induction of acute myeloid leukemia in mice by the human leukemia- specific fusion gene NUP98-HOXD13 in concert with Meis1. Blood 101, 4529-4538. Pineault, N., Helgason, C. D., Lawrence, H. J., and Humphries, R. K. (2002). Differential expression of Hox, Meis1, and Pbx1 genes in primitive cells throughout murine hematopoietic ontogeny. Exp Hematol 30, 49-57. |
5.10.7. BM transplantation and assessment of mice
For the bone marrow transplantation procedures, 8–10 week old recipient F1 (B6C3) mice were irradiated with 850 cGy from a 137Cs γ-radiation source. FACS purified transduced BM cells or defined ratios of transduced and unpurified and untransduced cells were injected into the tail vein of irradiated recipient mice. PB or BM cell progeny of transduced cells were tracked using GFP fluorescence (Feuring Buske, 2002). Lineage distribution was determined by flow cytometric analysis as previously described (Pineault et al., 2003). For histological analyses, sections of selected organs were prepared and H&E stained at the Academic Pathology Laboratory, GSF, Munich, using standard protocols. The mice were under observation for early signs of leukemia. Aspiration of the blood and bone marrow was performed at 8 weeks and subsequently at four week intervals and assessed for engraftment of the mice with transduced cells and progression of disease. The early signs of disease (leukemia) development were paleness of the feet, limited mobility or lethargy, shortness of breaths or ruffled body hair. Moribund mice were sacrificed and analyzed as described. Feuring-Buske M., Frankel A. E., Alexander R. L., Gerhard B., Hogge D. E. (2002). A diphtheria toxin-interleukin 3 fusion protein is cytotoxic to primitive acute myeloid leukemia progenitors but spares normal progenitors. Cancer Res. 62 (6), 1730-6. Pineault N., Buske C., Feuring-Buske M., Abramovich C., Rosten P., Hogge D. E., Aplan P. D., Humphries R. K. (2003). Induction of acute myeloid leukemia in mice by the human leukemiaspecific fusion gene NUP98-HOXD13 in concert with Meis1. Blood. 101 (11), 4529-38. |
The source is not mentioned. Note that there are two references "Pineault et. al., 2003" in the bibliography. |
|
Letzte Bearbeitung dieser Seite: durch Benutzer:Hindemith, Zeitstempel: 20140404230752